Fig. 1: Demethylation upregulated CD147 expression in NSCLC.

A MeDIP and hMeDIP analysis tracing for 5mC and 5hmC at the CD147 (BSG) locus. The square indicates that 5mC and 5hmC are located in the same region of the CD147 promoter. Genes were correlated to fit the UCSC annotations. B The content of 5mC and 5hmC in CD147 promoter were detected using ChIP-qPCR analysis in paired adjacent normal tissues and NSCLC tissues. *P < 0.05, Student’s t-test was used for statistical analysis. C Representative immunohistochemistry staining of CD147 in paired adjacent normal tissues and NSCLC tissues. D Representative methylation profiles of CpG dinucleotides in the CD147 promoter in adjacent normal tissues and NSCLC tissues detected using bisulfite genomic sequencing. CpG dinucleotides of CD147 promoter are expressed in roman numerals. Open circles indicate unmethylated CpG dinucleotides and closed circles indicate methylated CpG dinucleotides. The promoter methylation level of CD147 gene in a sample is expressed as the percentage of methylated CpG sites in all sequenced CpG sites shown in brackets. E Demethylation upregulates CD147 expression. CD147 expression was detected using real-time quantitative RT-PCR and western blotting in A549 cells after treatment with different concentrations of 5-Aza-dC. *P < 0.05, One-way ANOVA followed by Dunnett’s test was used for statistical analysis. F Genomic 5mC and 5hmC were detected in A549 cells using dot blot analysis after treatment with the indicated concentrations of 5-Aza-dC.