Fig. 3: TGF-β induced active demethylation of the CD147 promoter. | Oncogene

Fig. 3: TGF-β induced active demethylation of the CD147 promoter.

From: Active demethylation upregulates CD147 expression promoting non-small cell lung cancer invasion and metastasis

Fig. 3

A The CD147 expression was detected after treatment with 20 ng/mL TGF-β for various time periods. B CD147 promoter methylation status was examined using bisulfite genomic fsequencing. C Methyl-specific and unmethyl-specific PCR were performed using treated samples as described in (A). D ChIP-qPCR was used to detect the 5mC and 5hmC content of CD147 promoter after TGF-β treatment. The above experiments were carried out in A549 cells. *P < 0.05, **P < 0.01, one-way ANOVA followed by Dunnett’s test was used for statistical analysis in (C) and (D). E TGF-β induced the regulatory complex switch at the CD147 promoter. ChIP-qPCR was performed with the indicated antibodies after TGF-β treatment of A549 cells for 24 h. *P < 0.05, **P < 0.01, Student’s t-test was used for statistical analysis. F Interactions of KLF6-DNMT3A, KLF6-MeCP2, Sp1-Tet1, and Sp1-TDG in A549 cells treated with TGF-β were determined using co-IP assay.

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