Fig. 4: TGF-β stimulated active demethylation of CD147 by recruiting Tet1 and TDG. | Oncogene

Fig. 4: TGF-β stimulated active demethylation of CD147 by recruiting Tet1 and TDG.

From: Active demethylation upregulates CD147 expression promoting non-small cell lung cancer invasion and metastasis

Fig. 4

A and B Protein expression of Tet1, TDG, and CD147 was detected using western blotting after transfecting with Tet1 and TDG siRNA and the addition of TGF-β. Subsequently, the CD147 promoter methylation status was analyzed using bisulfite genomic sequencing. C Genomic 5mC and 5hmC levels were determined using dot blot analysis after treatment as described in (A). D AID and components of the BER machinery were recruited to the CD147 promoter detected by ChIP-qPCR using the indicated antibodies. The above experiments were carried out in NCI-H460 cells. **P < 0.01, Student’s t-test was used for statistical analysis.

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