Fig. 5: Targeted methylation of CD147 in NSCLC cells downregulated CD147 expression with dCas9-SunTag-DNMAT3A-sgCD147 system. | Oncogene

Fig. 5: Targeted methylation of CD147 in NSCLC cells downregulated CD147 expression with dCas9-SunTag-DNMAT3A-sgCD147 system.

From: Active demethylation upregulates CD147 expression promoting non-small cell lung cancer invasion and metastasis

Fig. 5

A Schematic description of targeted methylation via dCas9-SunTag-DNMAT3A-sgCD147. The sgRNAs recognizing respective target sites are shown in red. B CD147 expression was detected using quantitative real-time PCR after dCas9-SunTag-DNMAT3A-sgCD147 (1–6) transfection. C CD147 expression levels were detected using quantitative real-time PCR and western blotting after transfection using the dCas9-SunTag-DNMAT3A-sgCD147 system. DNMT3A expression was also detected using western blotting. *P < 0.05, **P < 0.01, one-way ANOVA followed by Dunnett’s test was used for statistical analysis in (A) and (B). D Subsequently, CD147 promoter methylation status was analyzed using bisulfite genomic sequencing.

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