Fig. 2: EWS cell lines showed a high sensitivity to selective G9a inhibition. | Oncogene

Fig. 2: EWS cell lines showed a high sensitivity to selective G9a inhibition.

From: Selective histone methyltransferase G9a inhibition reduces metastatic development of Ewing sarcoma through the epigenetic regulation of NEU1

Fig. 2

A Cell viability assessment by 50% inhibitory concentration (IC50) of 12 EWS cell lines, one non-tumor cell line, and 7 non-EWS cancer cell lines, after 72 h exposure to BIX01294. B Statistical comparison of the evaluation of G9a and H3K9me2 protein expression by immunoblotting in CADO-ES and RM82 EWS cell lines treated with IC50 or IC90 of BIX01294 at 24 and 48 h. GAPDH and histone 3 expression levels were used as internal controls. D Levels of cleaved-caspase 3 were assessed in CADO-ES and RM82 cells treated with BIX01294 in a dose-dependent manner for 48 h and analyzed by flow cytometry. E Apoptosis induction was evaluated through PARP cleaved protein quantification under BIX01294 treatment. F Expression levels of LC3-I and LC3-II autophagy proteins in EWS cell lines treated with BIX01294. Baf1A treatment was used as control of autophagy. G Analysis of autophagy biomarkers expression by RT-qPCR in EWS cell lines treated with BIX01294. *p < 0.05; **p < 0.01; ***p < 0.001.

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