Fig. 6: Inhibition of G9a via BIX01294 impairs metastases development in EWS spontaneous metastases mouse models. | Oncogene

Fig. 6: Inhibition of G9a via BIX01294 impairs metastases development in EWS spontaneous metastases mouse models.

From: Selective histone methyltransferase G9a inhibition reduces metastatic development of Ewing sarcoma through the epigenetic regulation of NEU1

Fig. 6

A, C Tumor growth was monitored in two spontaneous metastases mouse models upon and after BIX01294 therapy. Treatment ended after 21 days, and mice were followed until tumor volume reached 0.8–1 cm3. B, D Surgery time of mice as soon as the threshold was reached (control and BIX01294-treatment groups). Log-rank (Mantel-Cox) was used. E, F Quantification of Ki67-positively labeled nuclei, G9a strongly-positive tumor cells, and NEU1 protein expression after 21 days of BIX01924 treatment (upper panels). *p < 0.05; **p < 0.01; ***p < 0.001. Immunohistochemical staining of Ki67, G9a, and NEU1 in spontaneous metastases mouse model samples (20× and 40× magnifications). First column shows hematoxylin-eosin staining in treated or untreated tumors (lower panels). G, H Percentage of mice with or without the presence of lung metastases in EWS spontaneous metastases mouse models. I Significantly positive correlation by multivariate analysis (principal component and multivariate correlation) between G9a and number of metastases in EWS spontaneous metastases mouse models according to protein tumor expression. A negative correlation was observed between NEU1 expression and the two variables tested (G9a and number of metastases).

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