Fig. 2: IL-36R agonists increase the expression of cytokines/chemokines by colon cancer cells.

A Cells were stimulated with 100 ng/ml of IL-36α, IL-36β or IL-36γ. Changes in cytokine/chemokine expression were detected by qRT–PCR after 4 h and (B) by ELISA after 24 h. Data shown are mean ± s.e.m. (n = 3 independent experiments) and analysed by One-way ANOVA with Dunnetts post hoc test. p < 0.05, **p < 0.01.