Fig. 7: DUSP1 drives the pro-differentiation effect of CP-673451 treatment via inhibition of p38MAPK in U87 GBM cells and G179 GSCs.
From: PDGF-R inhibition induces glioblastoma cell differentiation via DUSP1/p38MAPK signalling
A, B Average length of neurite-like process per cell was determined for U87 GBM cells and G179 GSCs treated with DMSO, CP-673451, siCTRL DMSO or CP-673451, siDUSP1 DMSO or CP-673451 and BCI DMSO or CP-673451 for 24 h (ordinary one-way ANOVA). Representative images shown, red arrows indicating neurite-like processes. Images taken at 20x mag. Scale bar, 100 μm. C, D Western blotting analysis of phosphorylated and total SAPK/JNK, phosphorylated and total p44/p42MAPK, phosphorylated and total p38MAPK, DUSP1 and β-actin (loading control) in U87 GBM cells and G179 GSCs treated with DMSO or CP-673451 and BCI DMSO or CP-673451 for 24 h. Representative images shown. The mean ± SD of n = 3 independent experiments is shown *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 and ****p ≤ 0.0001 (ordinary one-way ANOVA).
