Fig. 2: TUFT1 promotes proliferation, migration and metastasis of HCC cells in vitro.

A Retrovirus encoding TUFT1 vector were transduced into Hep3B cells to establish Hep3B cells stably overexpressing TUFT1 (Hep3B-TUFT1 cells). Lentivirus encoding TUFT1 shRNA were transduced into HCCLM3 cells to establish HCCCLM3 cells with TUFT1 knockdown (HCCLM3-TUFT1 shRNA cells). Western blot was performed to confirm the overexpression or knockdown of TUFT1 in corresponding cells. n = three independent experiments, *P < 0.05 by t test. B MTT assay was performed to evaluate the effect of TUFT1 on the viability of Hep3B and HCCLM3 cells. n = three independent experiments, *P < 0.05 by two-way ANOVA. C BrdU assay was performed to evaluate the effect of TUFT1 on the proliferation of Hep3B and HCCLM3 cells. n = five randomly selected fields of three independent experiments, *P < 0.05 by t test. D 3D culture was used to investigate the effect of TUFT1 on growth of Hep3B and HCCLM3 cells. n = 30, *P < 0.05 by t test. E, F Transwell assasy was used to investigate the effect of TUFT1 on the migration and invasion of Hep3B and HCCLM3 cells. n = five randomly selected fields of three independent experiments, *P < 0.05 by t test.