Fig. 3: MYCN directly upregulates SMARCE1 expression by binding to the SMARCE1 promoter. | Oncogene

Fig. 3: MYCN directly upregulates SMARCE1 expression by binding to the SMARCE1 promoter.

From: SMARCE1 promotes neuroblastoma tumorigenesis through assisting MYCN-mediated transcriptional activation

Fig. 3

A Real-time qPCR analysis of MYCN and SMARCE1 expression upon MYCN shRNA knockdown in SK-N-BE(2)C and IMR32 cells. B Immunoblot detection of SMARCE1 protein levels after MYCN shRNA knockdown. C, D Relative luciferase activity of reporter constructs containing the SMARCE1 promoter in SK-N-BE(2) and IMR32 cells transfected with MYCN shRNA. E Upper panel, a total of five sets of primers were designed within the human SMARCE1 promoter; lower panel, ChIP-qPCR analysis using anti-IgG and anti-MYCN antibodies in SK-N-BE(2) cells. F ChIP-qPCR analysis using anti-IgG and anti-MYCN antibodies in IMR32 cells. G Predicted sequences of E-box in SMARCE1 promoter by JASPAR. H Luciferase assays performed using SMARCE1 wild-type and SMARCE1 mutant promoter. All data were shown as the mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, ns not significant.

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