Fig. 6: SMARCE1 and MYCN form co-activators that regulates PLK1, ODC1 and E2F2 transcription. | Oncogene

Fig. 6: SMARCE1 and MYCN form co-activators that regulates PLK1, ODC1 and E2F2 transcription.

From: SMARCE1 promotes neuroblastoma tumorigenesis through assisting MYCN-mediated transcriptional activation

Fig. 6

A–C Relative luciferase activity of reporter constructs containing the PLK1, ODC1 or E2F2 promoter in SK-N-BE(2) cells transfected with SMARCE1 shRNA. D Upper panel, a total of six sets of primers were designed within the human PLK1 promoter; lower panel, ChIP-qPCR analysis using anti-IgG and anti-SMARCE1 antibodies in SK-N-BE(2) cells. E, F Upper panel, a total of five sets of primers were designed within the human ODC1 or E2F2 promoter; lower panel, ChIP-qPCR analysis using anti-IgG and anti-SMARCE1 antibodies in SK-N-BE(2) cells. G ChIP analysis with MYCN and SMARCE1 antibodies upon SMARCE1 shRNA knockdown in SK-N-BE(2)C cells. H SK-N-BE(2) cells analyzed by re-ChIP to detect mutual binding of SMARCE1 and MYCN to promoters. The first round (initial ChIP) antibody (IgG or MYCN) is indicated below the plots and the second round (re-ChIP) antibody (IgG or SMARCE1) is shown in the color key. All data were shown as the mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, ns not significant.

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