Fig. 1: PrPC and Caveolin-1 relationship across cancer.
A Heatmap showing the top genes most positively and negatively correlated with the expression on PRNP in the CCLE. B qRT-PCR analysis of the relative expression of PRNP and CAV1 in PC3 prostate cancer cells versus PNT2 normal prostate cells (left panel); relative expression of PrPC and CAV1 mRNA (middle panel) or protein (right panel) in PRNP-silenced versus control PC3 prostate cancer cells. C qRT-PCR analysis of the relative expression of CAV1 PrPC-overexpressing versus control LoVo colon cancer cells (day 3 post-transfection) (left panel); relative expression of PrPC and CAV1 mRNA (middle panel) or protein (right panel) in PRNP-silenced versus control MDST8 colon cancer cells. Results are expressed as means of n = 2 independent triplicates of cell preparations ± s.e.m. *p < 0.05, ***p < 0.001 vs. control (Mann-Whitney test). Quantification for western blots is provided in Supplementary Fig. S2, with protein levels normalized to α-tubulin (α-tub). D Proximity ligation assay showing co-localization of PrPC and CAV1 in MDST8 cells (orange spots, right panel). Cells incubated with anti-PrPC antibody alone (left panel) or anti-Caveolin-1 antibody alone (middle panel) were used as controls. Nuclei were stained with DAPI.
