Fig. 2: PrPC controls Aβ levels in prostate and colon cancer cells.
A qRT-PCR analysis of the relative expression of APP and BACE1 in PC3 prostate cancer cells versus PNT2 normal prostate cells (left panel). Extracellular Aβ40 and Aβ42 levels were measured in the supernatants of PNT2 and PC3 cells (right panels). B APP and BACE1 mRNA levels, APP protein levels and extracellular Aβ40 and Aβ42 levels were measured in PRNP-silenced versus control PC3 prostate cancer cell extracts and supernatants. C APP and BACE1 mRNA levels, APP protein levels and extracellular Aβ40 and Aβ42 levels were measured in PRNP-silenced versus control MDST8 colon cancer cell extracts and supernatants. D APP and BACE1 mRNA levels and extracellular Aβ40 and Aβ42 levels were measured in PrPC-overexpressing versus control LoVo colon cancer cell extracts (day 3 post-transfection) and supernatants (days 3, 4 and 5 post-transfection). In B and C, protein levels were normalized to α-tubulin (α-tub). Results are expressed as means of n = 2 independent triplicates of cell preparations ± s.e.m. *p < 0.05, **p < 0.01 vs. control (Mann-Whitney test except for D middle and right panels Kruskal-Wallis and posthoc Wilcoxon rank-sum test with Holm’s correction). Quantification for western blots is provided in Supplementary Fig. S2C, D.
