Fig. 4: HDAC6 inhibition results in rapid MYC degradation.
From: Targeting the MYC interaction network in B-cell lymphoma via histone deacetylase 6 inhibition
A Western Blot analysis of Ramos cells treated for the indicated time with different concentrations of M-100. Levels of MYC were quantified to untreated conditions. Tubulin was used as a loading control. Cl. - cleaved. B Western Blot analysis of Ramos cells treated with 10 µM MG132 to block proteasomal degradation and/or 4 µM M-100 for the indicated time. Levels of MYC were quantified to untreated conditions. GAPDH was used as a loading control. C Ubiquitination of proteins was analyzed in Ramos cells treated for 3 h with 10 µM MG132, 4 µM M-100 or left untreated by Western blot. 50 µg protein was loaded for input. Endogenous MYC was immunoprecipitated from these cell lysates. Unspecific IgG was used for control IPs. Ub - Ubiquitin. D Western Blot analysis of B-cell lymphoma cell lines treated for 6 h and 24 h with 4 µM M-100. Vinculin was used as a loading control. Ac-tub - acetylated tubulin. Data in A–D are representative of n = 3 independent experiments.
