Fig. 2: Polarity and immune response gene signatures of IM and AM in the early and late stage of pulmonary metastasis. | Oncogene

Fig. 2: Polarity and immune response gene signatures of IM and AM in the early and late stage of pulmonary metastasis.

From: Evolving polarisation of infiltrating and alveolar macrophages in the lung during metastatic progression of melanoma suggests CCR1 as a therapeutic target

Fig. 2

A Macrophages were sorted from the lungs of C57BL/6 mice at d3 or d21 following i.v. B16F10 cell challenge. CD11b+CD11c- and CD11b-CD11c+ cell subsets back-gated on the F4/80+ cell population were identified as infiltrating (IM) and alveolar macrophages (AM), respectively, following cytospin preparations. B Quantification of IM and AM sorted from cell suspensions of unchallenged (control) lungs or from lungs at d3 or d21 after i.v. injection of B16F10 cells. The macrophage numbers are shown relative to the total cell counts of individual lungs (% of total events). Bars represent the means + SD, n = 4–6 per group. To assess the differences among the means, one-way ANOVA and Tukey’s post-hoc tests were performed, *p < 0.05, **p < 0.01. C–F Total RNA was isolated from pooled IM or AM of naïve or metastasis-bearing lungs (n = 5 per group) and subjected to a customised RT-qPCR array of mouse immune response and macrophage polarity genes. Expression of newly induced genes is displayed as average relative expression + SD, n = 3 (C, E). Relative expression of regulated genes in IM (D) and AM (F) at the early or late stage of lung metastasis is displayed as fold changes over the relative gene expression of IM and AM from unchallenged lungs. Means of changes of 5-fold or greater + SD are shown, n = 3.

Back to article page