Fig. 3: Effect of TLR activation on tumor cell growth, invasion and infiltration. | Oncogene

Fig. 3: Effect of TLR activation on tumor cell growth, invasion and infiltration.

From: Intralesional TLR4 agonist treatment strengthens the organ defense against colonizing cancer cells in the brain

Fig. 3

A 3D tumor cell spheroids (410.4 left, E0771-LG right) formed by the hanging-drop method. (A1) The spheroid area (in mm2) upon treatment with LPS (1 µg/ml) is depicted compared to the control (CTRL; mean ± SD, n ≥ 3; two-way ANOVA followed by Sidak’s multiple comparisons test). (A2) Representative images of spheroid outgrowth after 96 h are illustrated. Scale bars represent 500 µm. B 410.4 (left) or E0771-LG (right) tumor cell invasion after PBS (CTRL) or LPS (1 µg/ml) stimulation. Data represent the average tumor cell number migrated per high power field (HPF) after 96 h (n ≥ 12; mean ± SD; n.s. = not significant, ***p < 0.001; unpaired t-test). Organotypic brain-slice ex vivo co-cultures with 410.4 (C) or E0771-LG (D) tumor cells and stimulated with LPS (1 µg/ml), Pam3CSK4 (1 µg/ml), or 8-Br-cGMP (10 µM). Data represent the percentage degree of tumor-cell infiltration into the brain slices (WT) after 96 hours (n ≥ 14; n.s. = not significant, **p < 0. 01; Mann-Whitney test).

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