Fig. 3: Characteristics of Immune-enriched Subtype.

a Gene correlation network represents the hub genes of Immune-enriched Subtype identified by ARACNe method. Red dots stand for the top hub genes in the network. The dot’s size represents the relative expression level of each gene. b The gene-pathway co-expression network of Immune-enriched Subtype by the ARACNe and GSEA method. Dots in the center of the network stand for top hub genes, and the size of dots represents the relative expression level of each gene. Red dots and blue dots in the outer circle stand for the downstream upregulated and down regulated pathways of the hub genes, and dots’ size represents normalized enrichment score (NES). c Heatmap represents enrichment scores for immune signatures and gene expression levels in the Immune-enriched Subtype and other subtypes. d Box plots representing the relative expression of PD-1, PDL-1, CTLA4 and T cell inflamed GEP score in Immune-enriched Subtype and other subtypes. e The mIHC showed that several immune cells infiltration (CD8 + T cell, FoxP3+ Treg cells, CD86 + M1 macrophage and CD163 + M2 macrophage) in PTC tissues, BTN tissues and adjacent normal tissues. NES, normalized enrichment score; GEP, gene expression profile; mIHC, multiplexed immunohistochemistry.