Fig. 3: SCNN1B functions as a tumor suppressor in CRC cells via a cytokinetic effect. | Oncogene

Fig. 3: SCNN1B functions as a tumor suppressor in CRC cells via a cytokinetic effect.

From: The sodium channel subunit SCNN1B suppresses colorectal cancer via suppression of active c-Raf and MAPK signaling cascade

Fig. 3

A Overexpression of SCNN1B in DLD1 and SW1116 cells. B SCNN1B overexpression suppressed colony formation and C cell growth in DLD1 and SW1116 cells. D SCNN1B ectopic expression induced both early and late apoptosis in DLD1 cells, and promoted early apoptosis in SW1116 cells. E Western blot of apoptosis and cell cycle markers. SCNN1B increased cleaved caspase-8, caspase-9, caspase-7, and PARP indicative of apoptosis. SCNN1B suppressed Cyclin D1, whilst inducing p53, p21 and p27. SCNN1B inhibited MMP9, a marker for cell migration. F SCNN1B promoted G1-S cell cycle arrest in DLD1 and SW1116 cells, as evidenced by increased cells in G1 phase and decreased proportion of cells in S phase. G SCNN1B overexpression suppressed cell migration, as determined by wound healing assay. Values represent means ± S.E.M. *P < 0.05, **P < 0.01, ***P < 0.0001.

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