Fig. 2: Peritumoral MFs suppress, and intratumoral MFs promote, iCCA growth in vitro. | Oncogene

Fig. 2: Peritumoral MFs suppress, and intratumoral MFs promote, iCCA growth in vitro.

From: Spatiotemporal regulation of cholangiocarcinoma growth and dissemination by peritumoral myofibroblasts in a Vcam1-dependent manner

Fig. 2

A Alpha-SMA and Ki67 IHC in the 1-month and 3-month iCCA allograft tumors. Images in the same row share the scale bar of 100 μm. B Quantification of Ki67+ cells in the indicated areas of the 1- and 3-month iCCA allograft tumors. N/A: No core iMF-high regions present in the 1-month tumors. Three tumors were examined at each time point. Student t-test, P value *** <0.001, ****<0.0001. C Time course images of the ZsG+ SpheroidT and SpheroidT+iMF in the 2.5D coculture with the indicated bottom layers. All images share the same 200 μm scale bar. D Quantification of the diameters of SpheroidT in the indicated coculture conditions. Two-way ANOVA comparison, P value ****<0.0001. E Flow cytometry-based detection of the CellTracker-RFP dye of SpheroidT and SpheroidT+iMF placed on the indicated bottom layer. Note that RFP-negative (RFP-) cells are plotted as more RFP- cells indicates higher tumor cell proliferation index. P values of the Student t-test between the indicated groups are shown on the right.

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