Fig. 3: Hedgehog signaling and primary cilia formation in mouse osteosarcoma in vivo.
From: Ligand-dependent hedgehog signaling maintains an undifferentiated, malignant osteosarcoma phenotype
a Kaplan–Meier analysis of survival to ethical osteosarcoma endpoint in OsxCre (n = 29), OsxCre;Trp53lox/lox;Rb1lox/lox (Osx p53Rb KO; n = 21), OsxCre;p53lox/lox;Rb1lox/lox;Smolox/lox (Osx p53RbSmo KO, n = 19). P < 0.0001, Log-rank (Mantel-Cox) test. b X-ray images of a femoral osteosarcoma (arrow) in an Osx p53Rb KO and mandibular osteoid osteoma (arrow) in an Osx p53RbSmo KO mouse. Scale bar = 1 cm. Corresponding hemotoxylin and eosin stained sections are shown below. Scale bar = 30 µm. c SHH expression detected by immunohistochemistry in tumor sections from primary 45Ca and Osx p53Rb KO mouse osteosarcomas. Immunoperoxidase signal is shown in brown, counterstained with hematoxylin. Scale bar = 40 µm. d Immunofluorescence colocalization of acetylated α-tubulin (AcTUB) and ARL13b in primary 45Ca and Osx p53Rb KO mouse osteosarcoma tumor sections. Scale bar = 15 µm. e Primary cilia frequency in 45Ca and Osx p53Rb KO mouse osteosarcoma. n = 6 independent tumors, mean + SEM, *** P < 0.001, Student’s unpaired t test.
