Fig. 4: Hedgehog signaling and primary cilia formation in human osteosarcoma in vivo.
From: Ligand-dependent hedgehog signaling maintains an undifferentiated, malignant osteosarcoma phenotype
a SHH expression detected by immunohistochemistry in tumor sections of human osteosarcoma. Immunoperoxidase signal is shown in brown, counterstained with hematoxylin. Representative image is shown. Scale bar = 40 µm. b Quantification of SHH expression in human osteosarcoma tissue detected by immunohistochemical staining according to tumor grade. ** P < 0.01, one-way ANOVA/Tukey correction. c Quantification of SHH expression in human osteosarcoma tissue detected by immunohistochemical staining according to tumor subtype. **P < 0.005, ***P < 0.001, ****P < 0.0001, one-way ANOVA/Tukey correction. d Immunofluorescence colocalization of acetylated α-tubulin (AcTUB) and ARL13B human osteosarcoma tissue. Scale bar = 5 µm. e Relationship between SHH and the presence of primary cilia in human osteosarcoma tissue. N = 40. f Western blot analysis of SHH and ACTIN expression in human (U2OS, MG63) and mouse (D12M; Osx p53Rb KO) osteosarcoma cell lines (CL) and matched xenograft (X) and allograft (A) tissue. Lysate from NIH-3T3 cells is shown as a positive control. g SHH expression detected by immunohistochemistry in human osteosarcoma xenografts. Immunoperoxidase signal is shown in brown, counterstained with hematoxylin. Scale bar = 50 µm.
