Fig. 2: Arginase-1 is downregulated in tumor nodules from DDR2 KO mice. | Oncogene

Fig. 2: Arginase-1 is downregulated in tumor nodules from DDR2 KO mice.

From: DDR2-regulated arginase activity in ovarian cancer-associated fibroblasts promotes collagen production and tumor progression

Fig. 2

A Volcano plot showing differentially expresses genes in RNA from ID8TB−/− tumor nodules from Ddr2 WT mice (5 mice; n = 5) or Ddr2−/− mice (5 mice; n = 5). B Quantitative PCR assay showing Arg1 gene expression using RNA from ID8TB−/− (6 mice; n = 6) and BPPNM (3 mice; n = 3) tumor nodules from Ddr2 WT mice or Ddr2−/− mice. C Quantitative PCR assay showing Arg2 gene expression using RNA from ID8TB−/− and BPPNM tumor nodules from Ddr2 WT mice (3 mice; n = 3) or Ddr2−/− mice (3 mice; n = 3). D Arginase activity assay on tumor nodules from ID8TB−/− tumor-bearing Ddr2 WT mice (3 mice; n = 3) or Ddr2−/− mice (3 mice; n = 3). E Arginase activity assay on serum from ID8TB−/− tumor-bearing Ddr2 WT mice (6 mice; n = 6) or Ddr2−/− mice (6 mice; n = 6). F Immunohistochemistry images for Arginase-1 expression on ID8TB−/− tumor nodules from Ddr2 WT mice (6 mice; n = 6) or Ddr2−/− mice (6 mice; n = 6). Scale bars = 50 μm (left). One tumor slice per mouse (total of 6 tumor slices per group) was used and the entire tumor slice was analyzed using Halo. Graph on right shows percent of area positive for Arg1. In all panels, Student’s t-test was used for statistics ****p < 0.0001, ***p < 0.001, **p < 0.01.

Back to article page