Fig. 5: EIF4G2KD alters the transcriptomic and proteomic signatures of CD133+ population in HEC-1A cells. | Oncogene

Fig. 5: EIF4G2KD alters the transcriptomic and proteomic signatures of CD133+ population in HEC-1A cells.

From: Loss of EIF4G2 mediates aggressiveness in distinct human endometrial cancer subpopulations with poor survival outcome in patients

Fig. 5

Control and EIF4G2KD HEC-1A cells were FACS sorted by CD133 marker expression and subjected to RNA-seq and MS analysis. A Heat map showing hierarchical clustering of gene expression levels of all DEGs identified following RNA-seq analysis of CD133- and CD133+ sorted control and EIF4G2KD populations. B Heat map showing hierarchical clustering of all proteins with significantly changed abundance in MS analysis of CD133− and CD133+ sorted control and EIF4G2KD HEC-1A cells. C Venn diagram showing overlap of all differentially abundant proteins among the four comparisons. Numbers at the edges of the diagram represent the overall number of proteins with differential abundance in each comparison. D Volcano plot of the Log2 (Fold Ratio) of the abundance of the detected proteins in CD133+EIF4G2KD/CD133+Control comparison, vs. their significance expressed as log10 p-value. Proteins with significant increased abundance are indicated in orange, and decreased abundance in purple. E Total cell lysates from separated control and EIF4G2KD HEC-1A populations were subjected to western blot analysis for ALDH1A1 and GAPDH as loading control. Shown is a representative blot of 3 independent experiments. F High scoring significant pathways identified by GeneAnalytics pathway analysis of the set of proteins with increased abundance in the CD133+EIF4G2KD/CD133+Control comparison. Score numbers on X-axis indicate significance, numbers at right represent the number of proteins identified in the dataset out of the total number of proteins within the given pathway. G Protein expression of KLC1, KLC2 and KIF5 based on the abundance detected by the MS analysis. Data presented as mean of Log10 (Intensity), n = 4. Statistical significance was determined by two-way ANOVA (*p < 0.05, ***p < 0.001, ****p < 0.0001). Representative western blots of HEC-1A separated CD133− and CD133+ control and EIF4G2KD cells validating the MS results for KLC1, KIF5B, and KLC2. GAPDH was used as a loading control. Shown is one representative blot of n = 3.

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