Fig. 5: β-catenin modulates fibroblast transformation signalling via DKK4.
From: Colorectal cancer cells secreting DKK4 transform fibroblasts to promote tumour metastasis
A, B Western blot images (A) and quantification (B) of DKK4 by coimmunoprecipitation with LRP6 in MEFs cultured with CM obtained from the 1597-CCSCs. Statistical analysis was carried out with three technical replicates using each protein sample. C–E Western blot images (C) and quantification of the phosphorylation of β-catenin (Ser33/Ser37/Thr41) (D) and total β-catenin (E) in MEFs cultured with CM obtained from the 1597-CCSCs. Statistical analysis was carried out with three technical replicates using each protein sample. F–H Western blot images (F) and quantification of GSK3β (G) and ubiquitin (H) by coimmunoprecipitation with β-catenin in MEFs cultured with each CM obtained from the 1597-CCSCs and pretreated with 10 μM MG132 for 6 h. Statistical analysis was carried out with three technical replicates using each protein sample. I, J Representative images (I) and quantification (J) of F-actin (green) and α-SMA (red) double staining in MEFs cultured with (+) or without (-) WNT signalling modulators based on each CM obtained from 1597-CCSCs. MSAB, 2.5 μM; LiCl, 20 mM. Scale bars, 50 μm. MFI, mean fluorescence intensity. At least three independent experiments were performed. Mean ± SD, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ##p < 0.01; ###p < 0.001; ####p < 0.0001 by Student’s unpaired t test.
