Fig. 3: Genomic rearrangements and differential expression in cells with high PPM1D activity.
From: PPM1D activity promotes cellular transformation by preventing senescence and cell death
A Parental RPE-PPM1D-T2 cells and transformed RPE-PPM1D-T2-SA (clones 1 and 6) were arrested in mitosis, fixed and probed by M-FISH. Karyotyping of the remaining clones is shown in Suppl. Figure 2A. B Selected genes differentially expressed in parental RPE-PPM1D-T2 and transformed RPE-PPM1D-T2-SA cells. The heat map shows the differential expression normalised to nontransformed parental RPE-PPM1D-T2 cells. C Asynchronously growing parental RPE, RPE-PPM1D-T2 and transformed RPE-PPM1D-T2-SA clones 1–6 were lysed and expression of selected proteins was determined by immunoblotting. TFIIH and 14-3-3 were used as loading controls. Arrowhead indicates the position of PIK3IP protein. D Parental RPE, RPE-PPM1D-T2 and transformed RPE-PPM1D-T2-SA clones 1–6 were exposed or not to a high dose of IR (5 Gy), collected after 6 h and whole cell lysates were analysed by immunoblotting.
