Fig. 7: Comparison of HSC aging in different mouse strains.
From: Sex-dependent differences in hematopoietic stem cell aging and leukemogenic potential
a SP HSPCs in 27 to 28-month-old C57BL/6 mice vs 3-month-old controls in two sexes. Old females were divided into two groups with HSPCs% above (A) and below (B) the average. b SP compartment analysis for lower SP fractions within total SP cells. c Frequencies of highly enriched HSCs based on SP separation. d Frequencies of highly enriched HSCs based on LSK separation. e, f Comparison of BALB/c scRNA-seq data of this study with GSE59114 dataset for aging C57BL/6 and DBA/2 females, which was analyzed directly using standard RNA-seq data analysis pipelines without reclassifying the cells. Cluster dendrograms for Hallmark (e) and KEGG (f) aging pathways of LT-HSCs from three mouse strains were shown. g IFNα/γ responses from LT-HSCs vs mouse lifespan. C57BL/6, a long-lived strain, activated IFNα and γ responses. DBA/2, shorter-lived, only triggered IFNα. BALB/c females outlived males, activating both responses compared to males’ sole IFNγ activation. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant. The error bars represent one standard deviation.
