Fig. 6: Notch3 is functional in the initiation and progression of PDAC arising from Lfng-expressing cells.
From: Lfng-expressing centroacinar cell is a unique cell-of-origin for p53 deficient pancreatic cancer
A–D Immunostaining for Notch1 in the pancreas from p53fl/fl;Kras;Lfng-RFP/CreER (PKC-Lfng) and Lfngfl/fl;p53fl/fl;Kras;Lfng-RFP/CreER (LPKC-Lfng) mice at 28 and 45 days post tamoxifen injection. E–H Immunostaining for Notch2 in the PKC-Lfng and LPKC-Lfng pancreas at 28 and 45 days post-tamoxifen. I–L Immunostaining for Notch3 in the PKC-Lfng and LPKC-Lfng pancreas at 28 and 45 days post-tamoxifen. Arrows in I: positive staining in ADM. Arrowhead in I: positive staining in PanIN. M–P Immunostaining for Notch4 in the PKC-Lfng and LPKC-Lfng pancreas at 28 and 45 days post-tamoxifen. Q-Q” Double immunofluorescence staining for RFP (red) and Notch3 (green) in the PKC-Lfng pancreas at 28 days post-tamoxifen. Arrows: co-staining of RFP and Notch3 in centroacinar cells. R Anti-Notch3 immunostaining in the PKC-Lfng pancreas at 28 days post-tamoxifen. Arrow: positive staining in centroacinar cells. S-S” Double immunofluorescence staining for RFP (red) and Notch3 (green) in the PKC-Lfng pancreas at 45 days post-tamoxifen. T Kaplan-Meier survival analysis for PKC-Lfng and Nocth3-/-;p53fl/fl;Kras;Lfng-RFP/CreER (NPKC-Lfng) mice following tamoxifen induction. Log-rank test p = 0.0008. Scale bars: 50 μm.
