Fig. 5: Genomic copy number and protein expression of MCL1 in patients with OS.
From: Frequent copy number gain of MCL1 is a therapeutic target for osteosarcoma
A Copy number gain of MCL1 in 41 patients with OS. The ratio of MCL1 to 1p13.1 in 41 FFPE specimens from patients with OS was measured by the MCL1 FISH analysis. MCL1 gene gain is defined as MCL1/1p13.1 ratio ≥ 2.0, with high-level gain as MCL1/1p13.1 ratio ≥ 3.0 and low-level gain as MCL1/1p13.1 ratio ≥ 2.0 and < 3.0. B, C Examples of MCL1 FISH (B) and immunohistochemical (IHC, C) analyses in the specimens of patients with OS. Three specimens (GTA479-19, GTA525-19, and GTA476-30) defined as MCL1-amplified and one specimen (GTA525-3) defined as no gain is represented. An anti-Mcl-1 monoclonal antibody (CST, Cat#39224) is used for IHC staining (C). Scale bars: 20 μm.
