Fig. 1: AVJ16 binds to IGF2BP1 in cells.
CETSA was performed using H1299 cells. A Cells cultured for 3 h with either 10 μM AVJ16 or DMSO were subjected to the indicated temperature gradient for 3 min, lysed, and the soluble protein electrophoresed and analyzed on Western blots. Protein was visualized using antibodies specific for IGF2BP1, 2, or 3 (as indicated). The fraction of each IGF2BP paralog remaining intact was graphed as a function of temperature: B IGF2BP1; C IGF2BP2; D IGF2BP3. The r2 values for the curve fits (performed in GraphPad Prism) are shown for each of the curves. The Tm’s for the curves were calculated by determining the temperature at which 50% of the band had undergone degradation. Three biological repeats were performed.
