Fig. 2: Evaluation of RNA targets of IGF2BP1 using eCLIP.

eCLIP was performed using H1299 cells treated for 24 h with either DMSO or 1.5 µM AVJ16 in duplicate. A Pie charts showing the distribution of the peaks from DMSO (Non-treated) or AVJ16 (Treated) cells according to the region in the RNA to which they map. The table to the right shows the number of peaks and genes in which they are found. B Venn diagram showing the overlap of genes containing eCLIP peaks from DMSO and AVJ16-treated cells. C Tables showing the quantification of peaks and the genes in which they are found that were significantly up or down regulated as a result of AVJ16 treatment. The regions surrounding these differentially bound peaks (DBPs) were scaled to the same size (based on their transcription start site (TSS) and transcription end site (TES)) and calculated scores per genome region were plotted as a heat map, highlighting the peaks of the eCLIP compared to input, and comparing DMSO vs. AVJ16-treated peaks. D The number of genes (Y axis) with the indicated number of DBP peaks per gene (X axis) was plotted separately for the downregulated or upregulated DBPs. E In genes containing multiple DBPs, the large majority of genes (785/837 = 93.8%) are either all downregulated or all upregulated, as represented in the Venn diagram.