Fig. 6: AVJ16 induces apoptosis.
A–C Caspase 3/7 dye was added to cells treated with DMSO (black dots) or 4 μM AVJ16 (red dots) and monitored by the IncuCyte® S3 Live-Cell Analysis System. The amount of detected dye was normalized to cell number and plotted as a function of time. A H1299. B LKR-M-FL cells. C LKR-M-GFP. D, E, G, H, J, K FACS sorting to was used to detect Annexin/7-AAD-A staining of cells treated with DMSO or 4 μM AVJ16 for 48 h. Late apoptotic cells appear in the top right quadrant. The percent of late apoptotic cells in DMSO vs. AVJ16-treated cells was compared for H1299 cells F, LKR-M-FL cells I, and LKR-M-GFP cells L. *, p < 0.05; **, p < 0.01; ns not significant.
