Fig. 2: EBNA1 associates with MUC1-C in an auto-inductive pathway regulating EBNA1 and MUC1-C expression. | Oncogene

Fig. 2: EBNA1 associates with MUC1-C in an auto-inductive pathway regulating EBNA1 and MUC1-C expression.

From: MUC1-C auto-regulatory complex with EBNA1 is responsible for latent Epstein-Barr virus-associated gastric cancer progression

Fig. 2

A YCCEL1/tet-MUC1shRNA cells treated with vehicle or DOX for 14 days were analyzed for MUC1-C and EBNA1 transcripts by qRT-PCR. The results (mean±SD of four determinations) are expressed as relative levels compared to that obtained for vehicle-treated cells (assigned a value of 1). B YCCEL1/CshRNA and YCCEL1/MUC1shRNA#2 cells were analyzed for MUC1-C and EBNA1 transcripts by qRT-PCR. The results (mean±SD of four determinations) are expressed as relative levels compared to that obtained for CshRNA cells (assigned a value of 1). C YCCEL1/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days were analyzed for EBNA1 Qp transcripts (left). YCCEL1/empty vector and YCCEL1/EBNA1-DN cells were analyzed for EBNA1 Qp transcripts (right). The results (mean±SD of four determinations) are expressed as relative EBNA1 Qp mRNA levels compared to that obtained for vehicle-treated/empty vector cells (assigned a value of 1). D Lysates from YCCEL1/tet-MUC1shRNA cells treated with vehicle or DOX for 7, 10 and 14 days were immunoblotted with antibodies against the indicated proteins. E Lysates from YCCEL1/CshRNA and YCCEL1/MUC1shRNA#2 were immunoblotted with antibodies against the indicated proteins. F Lysates from YCCEL1 cells were precipitated with anti-MUC1-C or a control IgG. The precipitates and input lysate were immunoblotted with antibodies against the indicated proteins. G Lysates from AGS/EBNA1 cells were precipitated with anti-MUC1-C or a control IgG. The precipitates and input lysate were immunoblotted with antibodies against the indicated proteins. H Lysates from YCCEL1 cells were precipitated with anti-EBNA1 or a control IgG. The precipitates and input lysate were immunoblotted with antibodies against the indicated proteins. I Lysates from YCCEL1 cells expressing the designated vectors were treated with vehicle or DOX for 14 days and immunoblotted with antibodies against the indicated proteins.

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