Fig. 6: MUC1-C is necessary for the EBVaGC CSC state. | Oncogene

Fig. 6: MUC1-C is necessary for the EBVaGC CSC state.

From: MUC1-C auto-regulatory complex with EBNA1 is responsible for latent Epstein-Barr virus-associated gastric cancer progression

Fig. 6

A GSEA of RNA-seq data from YCCEL1 cells with MUC1-C silencing was performed using the BENPORATH ES1 gene signature. B Lysates from YCCEL1/CshRNA and YCCEL1/MUC1shRNA#2 cells were immunoblotted with antibodies against the indicated proteins. C Lysates from YCCEL1 cells treated with vehicle or 5 μM GO-203 for 4 days were immunoblotted with antibodies against the indicated proteins. D Lysates from YCCEL1/vector and YCCEL1/EBNA1-DN cells were immunoblotted with antibodies against the indicated proteins. Lysates from YCCEL1/tet-MUC1shRNA (E) and SNU-719/tet-MUC1shRNA (F) cells treated with vehicle or DOX for 7 days were immunoblotted with antibodies against the indicated proteins. G Lysates from YCCEL1/vector and YCCEL1/EBNA1-DN cells were immunoblotted with antibodies against the indicated proteins. H YCCEL1/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days were analyzed for tumorsphere formation. Shown are representative photomicrographs of tumorspheres (left). The results (mean±SD of three determinations) are expressed as relative tumorsphere formation compared to that for vehicle-treated cells (assigned a value of 1)(right). I YCCEL1/CshRNA and YCCEL1/MUC1shRNA#2 cells were analyzed for tumorsphere formation. Shown are representative photomicrographs of tumorspheres (left). The results (mean±SD of three determinations) are expressed as relative tumorsphere formation compared to that for CshRNA cells (assigned a value of 1)(right). J YCCEL1 cells treated with vehicle or 5 μM GO-203 for 7 days were analyzed for tumorsphere formation. Shown are representative photomicrographs of tumorspheres (left). The results (mean±SD of three determinations) are expressed as number of tumorsphers (right). K YCCEL1/vector and YCCEL1/EBNA1-DN cells were analyzed for tumorsphere formation. Shown are representative photomicrographs of tumorspheres (left). The results (mean±SD of three determinations) are expressed as relative tumorsphere formation compared to that for vector cells (assigned a value of 1) (right).

Back to article page