Fig. 1: σ1R controls stemness and tumorigenicity of prostate cancer cells.
From: Integrated control of cancer stemness by σ1 receptor in advanced prostate cancer
σ1R knockdown by shRNAs in DU145, PC3 and 22Rv1 cells by immunoblotting (A) and qRT-PCR (B). C Tumor-sphere formation and proliferation of σ1R-depleted (sh1_σ1R, sh2_σ1R) and control (sh_Control) cells. D–F Growth and weight of xenografts of σ1R-depleted and control DU145, PC3 and 22Rv1 cells. Data are mean ± SEM. Number of mice developing tumors and total number of mice injected per group are indicated. G Ex vivo tumor-sphere growth from control and σ1R-depleted DU145, PC3 and 22Rv1 xenografts. For 22Rv1 xenografts data with sh1 and sh2_σ1R shRNA are combined. Boxplots represent median, interquartile range, maximum and minimum. Data are mean ± SD; *P < 0.01 by t-test and ANOVA.
