Fig. 4: Slc7a5 is essential for CTL tryptophan uptake in tumor cell-activated CTLs in vitro. | Oncogene

Fig. 4: Slc7a5 is essential for CTL tryptophan uptake in tumor cell-activated CTLs in vitro.

From: Slc7a5 promotes T cell anti-tumor immunity through sustaining cytotoxic T lymphocyte effector function

Fig. 4

A–D 2/20 CTLs were co-cultured with 4T1 and AB1 tumor cells, respectively in the presence of JPH203 (10 μM) for 24 h, and analyzed for the indicated metabolites. E–H 2/20 CTLs were co-cultured with 4T1 and AB1 tumor cells, respectively, in the presence of JPH203 at the indicated doses for 6 h. The CTL and tumor co-culture mixtures were stained with CD8-specific mAb, Annexin V, and PI. CD8+ and CD8- cells were gated for cell death of the CTLs (E, F) and tumor cells (G, H).

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