Fig. 7: ECD protein is associated with mRNAs of glycolytic enzymes and regulates their stability. | Oncogene

Fig. 7: ECD protein is associated with mRNAs of glycolytic enzymes and regulates their stability.

From: ECD, a novel androgen receptor target promotes prostate cancer tumorigenesis by regulating glycolysis

Fig. 7

A, B Indicated PC cells were subjected to RNA immunoprecipitation using IgG or anti-ECD antibodies and processed as per instructions in Magna RNA RIP kit. Co-precipitated RNAs were purified and the indicated target RNAs were detected by qRT-PCR using gene specific primers. Graph display mean fold enrichment in each respective gene, as compared to IgG control. C–H mRNA stabilities of various glycolytic enzymes after actinomycin D (5 µg/mL) treatment for indicated time points in vector or ECD expressing PC cells. Trend lines were created by measuring relative abundance of each transcript with respect to the 0-min time point for vector and ECD-OE LNCaP and C4-2B cells and are expressed as percentage of mRNA remaining. Transcript abundance was measured by qRT-PCR using 18S rRNA as a housekeeping gene. Decay curves for indicated mRNAs are displayed for vector and ECD-OE cells and average half-life (t1/2) values are plotted as bar graphs. Quantitation data represent mean ± SEM with two-tailed unpaired t-test. n = 3; ***, p < 0.001.

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