Fig. 8: ECD overexpression increases glucose uptake and glycolytic rate. | Oncogene

Fig. 8: ECD overexpression increases glucose uptake and glycolytic rate.

From: ECD, a novel androgen receptor target promotes prostate cancer tumorigenesis by regulating glycolysis

Fig. 8

Glucose uptake in LNCaP (A) and C4-2B (B) PC cell lines. The values were normalized with respective to cell counts and depicted as compared with vector control. Quantification of results from three biological experiments, each with six technical replicates is shown as a bar graph. Data represents mean ± SEM and two-tailed unpaired Student's t-test (*p < 0.05, **p < 0.01, ***p < 0.001). C–J Sea horse glycolytic rate was assessed in vector and ECD-OE LNCaP (C) and C4-2B (E) cells. Cells were seeded in 96-well plates and exposed to Rot/AA (rotenone and antimycin cocktail) and 2-DG to measure proton efflux rate (PER, C, D) and extracellular acidification rate (ECAR, E, F). One representative PER and ECAR plots from three independent experiments are displayed (C–F). Basal glycolysis (G, H) and compensatory glycolysis (I, J) rates are presented as bar graphs, glycoPER in pmol/min calculated from subtracting ECAR (extra cellular acidification rate) from PER (proton efflux rates) (G–J). Quantitation data represent mean ± SEM with two-tailed unpaired t-test. n = 3; **, p < 0.01; ***, p < 0.001.

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