Fig. 2
From: MUC13 contributes to rewiring of glucose metabolism in pancreatic cancer
MUC13 regulates protein and mRNA expression of key factors involved in glucose metabolism. a Western blot showing the expression level of indicated proteins involved in glucose metabolism. Whole-cell lysates were collected from cells and the levels of protein expression were determined. b Densitometric analysis of bands for c-Myc, Glut-1 and HIF-1α. n = 2; *p < 0.05. c qPCR depicting changes in mRNA expression in MUC13-null (P-V/M-V) cells and MUC13-expressing (P-M13/M13) Panc-1 and MiaPaca cells; MUC13-expressing (sh-V) and knockdown (sh-M13) HPAF-II cells, *p < 0.05. d Semi-quantitative PCR performed to determine the expression of HIF-1 α and Glut-1 on MUC13 expression in PanCa cells and bars representing relative quantification of HIF-1α and Glut-1 expression. f Lactate assay performed in Panc-1 cells under normoxic and hypoxic conditions. *p < 0.05
