Fig. 3
From: MUC13 contributes to rewiring of glucose metabolism in pancreatic cancer
MUC13 expression leads to enhanced activation/nuclear translocation of NF-κB p65. a Western blots showing the expression level of p65 and phospho-p65 in M-V/M-M13 and P-V/P-M13 cells. Cells were spiked with TNF-α at a concentration of 20 ng/ml for 0, 10, and 30 min, and the nuclear extracts prepared. Histone H3 served as the internal control. b Western blots indicating changes in the expression of IκBα shown in the cytoplasmic extracts in aforementioned cell lines. c, d Western blots showing the expression level of p65, phospho-p65 and IκBα in the nuclear and cytoplasmic extracts of MUC13-expressing (sh-V) and MUC13 knockdown (sh-M13) HPAF-II cells. e Western blotting depicting the degradation of pIκB α and IκB α after treatment with MG132 (1 h, 10 µM) in presence of TNF-α stimulation (20 ng/ml, 30 min). f Western blotting depicting the effect of p65 silencing on the metabolism involved proteins in presence of TNF-α stimulation (5 ng/ml for 24 h) g Schematic representation depicting an activation of NFҡB on IҡB phosphorylation in presence of MUC13 following TNF-α or okadaic acid stimulation. This triggers NFҡB translocation to the nucleus and induction of target gene expression
