Fig. 4
From: MUC13 contributes to rewiring of glucose metabolism in pancreatic cancer
MUC13 interacts with Glut-1. a, b Reciprocal co-immunoprecipitation assay performed between MUC13 and Glut-1 using whole-cell protein lysates from MUC13-expressing, AsPC1, and HPAF-II cells. c Cycloheximide chase assay depicting the stability of Glut-1 in P-V and P-M13 cells. Cells were treated with cycloheximide (10 μM) at different indicated time points followed by lysate preparation and Western blotting. Alpha-tubulin (α-tubulin) served as an internal control. d Bars representing relative quantification of the blots. e In situ proximity ligation assay (PLA) performed using HPAF-II cells with the Duolink Red starter kit. DAPI was used as a counter stain for the nucleus. MUC13/Her2 served as positive control, and MUC13 alone as negative control. The co-localization between MUC13/Glut-1 and MUC13/Her2 has been indicated by arrows. f Co-capping assay to demonstrate interaction between MUC13 and Glut-1 in HPAF-II and AsPC1 cells. HPAF-II cells with anti-α tubulin is a negative control for this experiment. Images were captured at ×400
