Fig. 5: Tumor-derived exosomes promote PD1+ macrophages expansion from monocytes in vitro and in vivo. | Oncogenesis

Fig. 5: Tumor-derived exosomes promote PD1+ macrophages expansion from monocytes in vitro and in vivo.

From: Tumor-derived exosomes induce PD1+ macrophage population in human gastric cancer that promotes disease progression

Fig. 5

a Isolated exosomes were characterized by Nano-sight, western blot, and flow cytometry. b–d On day 3, monocytes cultured with or without exosomes were evaluated by flow cytometry to assess the frequency of PD1, and CD206 in monocytes. Representative FACS plots are shown in (c). b, d Results represent the mean of three independent experiments. e–g Analysis of IL-10 (e), IL-12p70 (g), and CCL1 (f) expression in monocytes cultured with or without exosomes by ELISA. Results represent the mean of three independent experiments. h Phagocytic ability analysis of monocytes cultured with or without exosomes by flow cytometry. Results represent the mean of three independent experiments. i–l Suppressive ability analysis of monocytes cultured with or without exosomes by flow cytometry. Results represent the mean of three independent experiments. m On day 3, monocytes cultured in the presence of exosomes that were collected from SGC7901 with or without the exosome release inhibitor spiroepoxide were evaluated by flow cytometry to assess the frequency of PD1. n, o FACS analysis of PD1 expression on macrophages from the samples as follows: non-tumoral tissue and tumor tissue from tumor-bearing mouse. Representative FACS plots of macrophage PD1 expression are shown in (n). Frequencies of PD1+ macrophages relative to total macrophage frequencies are shown in (o)

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