Fig. 6: HBx promoted HCC cell invasion through ECM modification. | Oncogenesis

Fig. 6: HBx promoted HCC cell invasion through ECM modification.

From: Hepatitis transactivator protein X promotes extracellular matrix modification through HIF/LOX pathway in liver cancer

Fig. 6

a MHCC97L and Hep3B HBx knockdown HCC cells and their control cells were exposed to 20 and 1% O2 for 48 h. Conditioned media were collected and incubated with matrigel that was coated on the Transwell chambers. Conditioned media were removed. Parental HCC cells in serum free conditioned media were seeded onto modified ECM coated Transwell chambers and allowed to invade for 24 h. b, c Left: Parental HCC cells invaded through the matrigel-coated Transwells that were modified with conditioned media from the indicated HCC subclones. Right: number of invadesd cells were counted in at least three random fields. *p < 0.05, **p < 0.01, ***p < 0.001, Student’s t test

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