Fig. 2: Role of PDGFR-α/β in sarcoma CSCs. | Oncogenesis

Fig. 2: Role of PDGFR-α/β in sarcoma CSCs.

From: RETRACTED ARTICLE: Platelet-derived growth factor receptor-α and -β promote cancer stem cell phenotypes in sarcomas

Fig. 2

a Western blot analysis of phosphorylated and total PDGFR-α and -β in human sarcoma cell lines grown as monolayers and as spheroids. β-actin was used as a loading control. b Western blot analysis of PDGFR-α and -β expression and self-renewal transcription factors in human sarcoma cell lines grown as spheroids and treated with imatinib (1 μM) or DMSO control. Experiments in a, b were performed three times with similar results. c Graph displaying the mean diameters of the spheroids for HT1080, SK-LMS-1, and DDLS8817 cells treated with imatinib or DMSO. d Light microscopy images from single cell assays for spheroid cells treated with imatinib or DMSO. e Representative light microscopy images from spheroid formation assays performed in triplicate of human sarcoma cell lines under varying conditions. Cells were grown as spheroids following PDGFR-α and PDGFR-β double knockdown (sh.PDGFR-α/β), transduction with scramble shRNA (sh.Scr), or treatment with imatinib or DMSO. Graphs display the mean numbers of spheroids ≥50 μm per field in each treatment group. Bars represent standard deviation. *p < 0.05 compared to DMSO

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