Fig. 3: PDGFR-α/β promotes sarcoma CSC migration/invasion and anchorage-independent growth. | Oncogenesis

Fig. 3: PDGFR-α/β promotes sarcoma CSC migration/invasion and anchorage-independent growth.

From: RETRACTED ARTICLE: Platelet-derived growth factor receptor-α and -β promote cancer stem cell phenotypes in sarcomas

Fig. 3

a Western blot analysis of cell adhesion protein N-cadherin and EMT transcription factors Snail, Slug, and Zeb1 in human sarcoma cell lines grown as monolayers and as spheroids. β-actin was used as the loading control. b Representative images under light microscopy of migration and invasion assays of human sarcoma cell lines grown as monolayers or as spheroids for 24–48 h. Graphs display the number of migrated or invasive cells per field. c Representative light microscopy images of forrmed colonies in soft agar assay of human sarcoma cell lines grown as monolayers or spheroids for 14–20 days. Graph displays the number colonies per field. d Representative images under light microscopy of migration and invasion assays of human sarcoma cell lines grown as monolayers or as spheroids. Spheroid cells were treated with imatinib or DMSO. e Western blot analysis of N-cadherin and EMT-regulating transcription factors in human sarcoma cell lines grown as spheroids and treated with imatinib or DMSO. Experiments in a and e were performed three times with similar results. Bars represent standard deviation. *p < 0.05 compared to Monolayers

Back to article page