Fig. 4: Effect of Otx2 ablation on Shh signalling pathway and cell cycle gene expression. | Oncogenesis

Fig. 4: Effect of Otx2 ablation on Shh signalling pathway and cell cycle gene expression.

From: Otx2 promotes granule cell precursor proliferation and Shh-dependent medulloblastoma maintenance in vivo

Fig. 4

a Regulation of Shh signalling pathway effector genes upon Otx2 ablation. Expression of the indicated genes was monitored using real-time quantitative PCR and normalized to GAPDH expression. Results are displayed as the fold change between sKO and control cerebella at P3 or P5. None of the tested genes were significantly modified (p > 0.05). b Relative expression of the cell cycle inhibitors Cdkn2d (p19), Cdkn1b (p27) and Cdkn1c (p57) in Otx2 sKO and control cerebella at P1, P3 and P5 indicate upregulation upon Otx2 ablation. c Cell cycle analysis of Otx2+ and Otx2− GCPs. GCPs were purified from Otx2+/Otx2-GFP cerebella at P7 and analysed by FACS, as in Fig. 1d. Proliferating populations were identified by Ki67 staining and their DNA content was analysed using the DNA-intercalating FxCycleTM fluorescent dye. d Distribution of Otx2+ and Otx2− GCPs in each phase of the cell cycle was calculated using the Modfit programme. P postnatal day, sKO self-knockout. Data are displayed as mean ± sem. *p < 0.05, **p < 0.01, ***p < 0.001

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