Fig. 2: Overexpression and knockdown of LGR5 have opposite effects on cell proliferation. | Oncogenesis

Fig. 2: Overexpression and knockdown of LGR5 have opposite effects on cell proliferation.

From: LGR5 regulates gastric adenocarcinoma cell proliferation and invasion via activating Wnt signaling pathway

Fig. 2

a, c Gastric adenocarcinoma cells were plated 24 h after transfection with 1.5 × 103 cells/well in six-well plates for 14 days then tested for their ability of clonogenicity as described in Methods. The colonies (≥50 cells) were numbered. Representative images of colonies in plates stained with Giemsa. Images were taken using a Nikon 90i with a DXM 1200C camera. b, d Data are presented as values of mean ± SD from three independent measurements and the asterisk indicates statistical significance compared with the control (untransfected) parental cells. P-values were calculated with Student’s t test. **P < 0.01. e, f Cell viability was assessed by MTT assay. Cell growth curves were determined by absorbance at 570 nm. Experiments were done in triplicate and data from three independent experiments are shown. **P < 0.01: error bars show SEM

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