Fig. 5: LGR5 regulates proliferation and invasion through Wnt/β-catenin signaling in BGC823 and SGC7901 cells.

Cells were transiently transfected with corresponding plasmids. Meanwhile, LGR5 overexpressing cells were treated with Wnt inhibitor C59 and LGR5-knowdown cells were treated with activator Wnt3a. Twenty-four hours after treatment, the cells were examined in the wound healing assay. MTT assays were also started 24 h after treatment. MTT assay (a, b) and wound healing assay (c–f) were performed as described in Methods. **P < 0.01