Fig. 5: RecQL4 deficiency induced G2/M cell cycle arrest and apoptosis. | Oncogenesis

Fig. 5: RecQL4 deficiency induced G2/M cell cycle arrest and apoptosis.

From: RecQL4-Aurora B kinase axis is essential for cellular proliferation, cell cycle progression, and mitotic integrity

Fig. 5

a Apoptotic induction by RecQL4 depletion that was reversed by wild-type RecQL4 reconstitution analyzed by Annexin V + PI staining. To quantify the percentage of Annexin V + PI-positive cells, a total of over 300 cells in six randomly selected fields were counted under a epifluorescence microscope, and the number of positive cells (Annexin V + PI staining) was recorded. Data obtained from three independent experiments are presented as mean percentage ± SD. One-way ANOVA statistical analysis, **p value <0.01. b Percentage of the G2/M-phase cells in RecQL4-deficient U2OS cells with or without AURKB reconstitution were measured by PI staining and by flow cytometer at 72 h post transfection. Forced expression of AURKB in RecQL4-suppressed U2OS cells showed the attenuation of cells at the G2/M phase. Data represent the mean ± SD from three independent experiments. One-way ANOVA statistical analysis, **p value <0.01

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