Fig. 1: Establishment of vagotomy in an orthotopic xenograft nude mouse model.
From: Muscarinic acetylcholine receptor 3 mediates vagus nerve-induced gastric cancer
a Injection of luciferase-labelled MGC-803 cells into the subserosa of the lesser curvature of the mouse stomach. b Formation of a xenograft tumour in a mouse stomach (red arrow points to a xenograft tumour). c Transection of the left trunk of the vagal nerve at the subdiaphragmatic level. d Immunohistochemical (IHC) analysis of PGP9.5 in the dissected vagus nerve (original magnification, ×100; scale bar = 100 μm). e Haematoxylin and eosin staining (HE) of mouse gastric mucosa in the sham and vagotomy groups (original magnification, ×100; scale bar = 100 μm). f Average thickness of mouse gastric mucosa in the sham and vagotomy groups. g Body weight of the mice in the sham and vagotomy groups
