Fig. 5: Confirmation of the role of M3 in vagus nerve-induced GC.

a qRT-PCR analysis of M1, M2, M3, M4 and M5 mRNA in stomach tissues of the sham and vagotomy groups. b IHC of M3 in stomach tissues of the sham and vagotomy groups (original magnification, ×100; scale bar = 100 μm). c Scatter plot showing IHC scores for M3 protein expression in stomach tissues of the sham and vagotomy groups. d IHC of ChAT in stomach tissues of the sham and vagotomy groups (original magnification, ×100; scale bar = 100 μm). e Scatter plot showing IHC scores for ChAT protein expression in stomach tissues of the sham and vagotomy groups. f Tumour radiance on the indicated days in the control and darifenacin groups. g Tumour weights in the control and darifenacin groups. h Box plot showing IHC scores for M3 protein expression in 120 human GC tissues and their matched non-cancerous gastric tissues. i IHC of M3 in human GC tissues and their matched noncancerous gastric tissues (original magnification, ×200; scale bar = 100 μm)